While we haven't analyzed PneumaCult™ media to be used with murine epithelial cells, there are actually publications that report this application.
The subsequent illustration is for getting ready RNP complexes for one reaction. Regulate appropriately according to the amount of reactions expected.
Stream cytometry can be used to evaluate the performance of differentiation protocols with the STEMdiff™ system, by thinking about particular marker expression at Just about every stage of your differentiation method. It can even be used for other apps, such as cell sorting, immunophenotyping, and purity evaluation.
If cells usually do not dissociate nicely with trituration, go for a longer ACCUTASE™ incubation, as much as 12 minutes. If monolayers do not dissociate soon after twelve minutes of ACCUTASE™ incubation and trituration, the differentiation was very likely unsuccessful.
Cross-part histology followed by hematoxylin and eosin (H&E) staining to assess the thickness on the smaller or substantial airway epithelium
For finest effects, the whole volume of cargo added shouldn't exceed ten% on the reaction volume. Decreasing the response quantity to less than eighty µL may well cause reduce enhancing efficiencies and cell recoveries.
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Here is a summary of revealed literature 줄기세포 지방이식 that cites culturing of nasal epithelial cells working with PneumaCult™ culture media:
Notes: STEMdiff™ Kidney Organoid Kit consists of a special plate structure/tradition established-up and isn't an average monolayer society; kidney organoids protrude from your fundamental monolayer tradition.
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Indeed, you’ll locate the step-by-move protocol for 줄기세포 지방이식 TEER measurement to evaluate the epithelial barrier integrity in ALI cultures listed here.
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